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A Modification of the Revolving Mirror Method for Measuring by Michelson A.A.

By Michelson A.A.

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Extra resources for A Modification of the Revolving Mirror Method for Measuring the Velocity of Light (1920)(en)(2s)

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Without moving the stage, image the same ROI under a Blue filter to acquire fiducial fluorescence. Given that regions between grid bars where Formvar/ sections are unsupported and are therefore, not flat, often only part of the ROI will be in focus. Try to acquire several images in the same ROI in the same epifluorescence channel using different focusing steps to ensure that as many areas within the ROI are in focus. Ensure that you have acquired all relevant fluorescent channels before moving the stage to seek out a new ROI.

1 Strategies for CLEM correlation between LM and EM. , 2014), having fluorescent fiducials that, when quenched, were mostly fluorescing in only the UV-blue channel was optimal. Second, the 200 nm polystyrene-based Blue FluoSpheres® were of sufficient electron density to be easily observed via electron microscopy rendering them useful in aligning areas of interest in TEM with LM images. 4 NA) oil immersion lens with an upright epifluorescence microscope at excitation/emission of 500/520 for blue, for the fiducials, and 488/514 for GFP (Fig.

Transformed yeast cells were freeze-substituted and embedded in HM20 resin. , <120 nm thick) and sections thicker than 150 nm will be difficult to resolve by TEM unless electron tomography is used. 1 Strategies for CLEM correlation between LM and EM. , 2014), having fluorescent fiducials that, when quenched, were mostly fluorescing in only the UV-blue channel was optimal. Second, the 200 nm polystyrene-based Blue FluoSpheres® were of sufficient electron density to be easily observed via electron microscopy rendering them useful in aligning areas of interest in TEM with LM images.

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